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PRMT5 antibody [GT1142]

Recombinant antibody
Anti-PRMT5 antibody [GT1142] used in Western Blot (WB). GTX00832
Anti-PRMT5 antibody [GT1142] used in IHC (Paraffin sections) (IHC-P). GTX00832
Anti-PRMT5 antibody [GT1142] used in IHC (Paraffin sections) (IHC-P). GTX00832
Anti-PRMT5 antibody [GT1142] used in Western Blot (WB). GTX00832

Cat. No. GTX00832

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1142

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PRMT5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan PRMT5

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 5 , HRMT1L5 , HSL7 , IBP72 , JBP1 , SKB1 , SKB1Hs

Cellular Localization

Cytoplasm,Nucleus,Golgi apparatus

Background

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PRMT5 antibody [GT1142] used in Western Blot (WB). GTX00832

GTX00832 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT1142] (GTX00832) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRMT5 antibody [GT1142] used in IHC (Paraffin sections) (IHC-P). GTX00832

GTX00832 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00832 PRMT5 antibody [GT1142].
Dlution : 1:100

Anti-PRMT5 antibody [GT1142] used in IHC (Paraffin sections) (IHC-P). GTX00832

GTX00832 IHC-P Image

IHC-P analysis of rat testis tissue section using GTX00832 PRMT5 antibody [GT1142].
Dlution : 1:100

Anti-PRMT5 antibody [GT1142] used in Western Blot (WB). GTX00832

GTX00832 WB Image

WB analysis of various samples using GTX00832 PRMT5 antibody [GT1142].
Dilution :
Loading : 25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399