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PRMT5 antibody [GT3610]

Anti-PRMT5 antibody [GT3610] used in Immunoprecipitation (IP). GTX628488
Anti-PRMT5 antibody [GT3610] used in Western Blot (WB). GTX628488
Anti-PRMT5 antibody [GT3610] used in Western Blot (WB). GTX628488

Cat. No. GTX628488

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3610

Isotype

 IgG2a

Application

 WB, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PRMT5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , NCI-H929 , rat kidney

Predict Reactivity

Mouse, Zebrafish, Bovine, Pig, Japanese Medaka, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT5. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888041

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 5 , HRMT1L5 , HSL7 , IBP72 , JBP1 , SKB1 , SKB1Hs

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES

Anti-PRMT5 antibody [GT3610] used in Immunoprecipitation (IP). GTX628488

GTX628488 IP Image

PRMT5 antibody [GT3610] immunoprecipitates PRMT5 protein in IP experiments.
IP samples: Raji whole cell extract
A. 35 μg Raji whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PRMT5 protein by 4 μg PRMT5 antibody [GT3610] (GTX628488)
7.5 % SDS-PAGE
The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [GT3610] (GTX628488) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-PRMT5 antibody [GT3610] used in Western Blot (WB). GTX628488

GTX628488 WB Image

PRMT5 antibody [GT3610] detects PRMT5 protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
7.5% SDS-PAGE
PRMT5 antibody [GT3610] (GTX628488) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PRMT5 antibody [GT3610] used in Western Blot (WB). GTX628488

GTX628488 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (GTX628488) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Yang M et al. Cell Rep 2020; 30 (12) : 4165-4178.e7 OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations