PRMT5 antibody [N1C1]

Cat. No. GTX116004

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP, ChIP assay
Reactivity	Human, Mouse
Package	100 μl ($419), 25 μl ($169)

See all PRMT5 products

View product citations for antibody GTX116004 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624553

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 5 , HRMT1L5 , HSL7 , IBP72 , JBP1 , SKB1 , SKB1Hs

Background

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.

Database

Gene ID: 10419 PRMT5
UniProt: O14744 PRMT5

Research Area

DATA IMAGES

GTX116004 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5(GTX116004) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116004 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [N1C1] (GTX116004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116004 IHC-P Image

PRMT5 antibody [N1C1] detects PRMT5 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PRMT5 stained by PRMT5 antibody [N1C1] (GTX116004) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX116004 IP Image

PRMT5 antibody [N1C1] immunoprecipitates PRMT5 protein in IP experiments.
IP samples: Raji whole cell extract
A. 35 μg Raji whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PRMT5 protein by 4 μg PRMT5 antibody [N1C1] (GTX116004)
7.5 % SDS-PAGE
The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] (GTX116004) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX116004 ChIP assay Image

Cross-linked ChIP was performed with K562 chromatin extract and 5 μg of either control rabbit IgG or anti-PRMT5 antibody. The precipitated DNA was detected by PCR with primer set targeting to γ-globin promoter.
ChIP experiment and primer designs are based on Nat Struct Mol Biol. 2009 Mar;16(3):304-11