PRMT5 antibody [N1N3]

Cat. No. GTX115994

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all PRMT5 products

Datasheet
Document

View product citations for antibody GTX115994 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PRMT5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622111

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 5 , HRMT1L5 , HSL7 , IBP72 , JBP1 , SKB1 , SKB1Hs

Cellular Localization

Cytoplasm , Nucleus

Database

Gene ID: 10419 PRMT5
UniProt: O14744 PRMT5

Research Area

DATA IMAGES

GTX115994 IHC-P Image

PRMT5 antibody [N1N3] detects PRMT5 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PRMT5 antibody [N1N3] (GTX115994) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115994 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRMT5(GTX115994) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115994 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX115994 diluted at 1:1000

GTX115994 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PRMT5(GTX115994) antibody at 1:500 dilution.

GTX115994 IHC-P Image

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

PRMT5 antibody [N1N3]

Cat. No. GTX115994

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX115994 IHC-P Image

GTX115994 IHC-P Image

GTX115994 WB Image

GTX115994 ICC/IF Image

GTX115994 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.