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PRMT6 antibody, Internal

Anti-PRMT6 antibody, Internal used in ChIP assay (ChIP assay). GTX47433
Anti-PRMT6 antibody, Internal used in Western Blot (WB). GTX47433

Cat No. GTX47433

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ChIP assay

Reactivity

Human
Package
50 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human PRMT6

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein arginine methyltransferase 6 , HRMT1L6

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-PRMT6 antibody, Internal used in ChIP assay (ChIP assay). GTX47433

GTX47433 ChIP assay Image

ChIP analysis of HCT116 cells using GTX47433 PRMT6 antibody. Use 0.5μg antibody GTX47433 PRMT6 antibody for ChIP assay. Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Anti-PRMT6 antibody, Internal used in Western Blot (WB). GTX47433

GTX47433 WB Image

WB analysis of 293T cells using GTX47433 PRMT6 antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf