PRMT7 antibody

PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 μg of PRMT7 antibody (GTX116570) 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody (GTX116570) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PRMT7 antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT7 protein stained by PRMT7 antibody (GTX116570) diluted at 1:500.
Blue: Hoechst 33342 staining.

Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 μg of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.