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PROX1 antibody

Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Western Blot (WB). GTX129143
Anti-PROX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129143

Cat No. GTX129143

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

HepG2 nuclear extract , mouse hippocampus , mouse eye , fetal mouse forebrain

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PROX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prospero homeobox 1

Cellular Localization

Nucleus

Background

May play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons.

Database

Research Area

DATA IMAGES

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

HepG2 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 antibody (GTX129143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 antibody (GTX129143) diluted at 1:500.

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PROX1 antibody (GTX129143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PROX1 antibody used in Western Blot (WB). GTX129143

GTX129143 WB Image

PROX1 antibody detects PROX1 protein by western blot analysis.
A. 50 μg fetal mouse forebrain lysate/extract
7.5 % SDS-PAGE
PROX1 antibody (GTX129143) dilution: 1:1000

Anti-PROX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129143

GTX129143 ICC/IF Image

PROX1 antibody detects PROX1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PROX1 protein stained by PROX1 antibody (GTX129143) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for PROX1 antibody (GTX129143). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PROX1 antibody (GTX129143). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169