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PRP19 antibody [N2C2], Internal

Anti-PRP19 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111233
Anti-PRP19 antibody [N2C2], Internal used in Western Blot (WB). GTX111233
Anti-PRP19 antibody [N2C2], Internal used in Western Blot (WB). GTX111233
Anti-PRP19 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111233

Cat. No. GTX111233

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRP19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

H1299 , HeLa , HepG2 , Mouse stomach

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRP19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951466

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pre-mRNA processing factor 19 , NMP200 , PRP19 , PSO4 , SNEV , UBOX4 , hPSO4

Cellular Localization

Nucleus , nucleoplasm

Background

PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PRP19 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111233

GTX111233 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRP19(GTX111233) antibody at 1:200 dilution.

Anti-PRP19 antibody [N2C2], Internal used in Western Blot (WB). GTX111233

GTX111233 WB Image

Sample (50 ug of whole cell lysate)
A: mouse stomach
7.5% SDS PAGE
GTX111233 diluted at 1:1000

Anti-PRP19 antibody [N2C2], Internal used in Western Blot (WB). GTX111233

GTX111233 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX111233 diluted at 1:1000

Anti-PRP19 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111233

GTX111233 IHC-P Image

PRP19 antibody [N2C2], Internal detects PRP19 protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
PRP19 antibody [N2C2], Internal (GTX111233) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169