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PRP8 antibody [C3-2], C-term

Anti-PRP8 antibody [C3-2], C-term used in Western Blot (WB). GTX108046

Cat. No. GTX108046

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PRP8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

274 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRP8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241213

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pre-mRNA processing factor 8 , HPRP8 , PRP8 , PRPC8 , RP13 , SNRNP220

Cellular Localization

Nucleus speckle

Background

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRP8 antibody [C3-2], C-term used in Western Blot (WB). GTX108046

GTX108046 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
5% SDS PAGE
GTX108046 diluted at 1:500

REFERENCE

Application Reference

Moriel-Carretero M et al. J Cell Biol 2017; 216 (12) : 4007-4026 Fanconi anemia FANCD2 and FANCI proteins regulate the nuclear dynamics of splicing factors.
Ameyar-Zazoua M et al. Nat Struct Mol Biol 2012; 19 (10) : 998-1004 Argonaute proteins couple chromatin silencing to alternative splicing.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169