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PRP8 antibody

Anti-PRP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX65909
Anti-PRP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX65909
Anti-PRP8 antibody used in Western Blot (WB). GTX65909
Anti-PRP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX65909
Anti-PRP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65909

Cat. No. GTX65909

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PRP8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

274 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pre-mRNA processing factor 8 , HPRP8 , PRP8 , PRPC8 , RP13 , SNRNP220

Cellular Localization

Nucleus speckle

Background

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PRP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX65909

GTX65909 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX65909 PRP8 antibody.
Dilution : 1:100

Anti-PRP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX65909

GTX65909 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX65909 PRP8 antibody.
Dilution : 1:100

Anti-PRP8 antibody used in Western Blot (WB). GTX65909

GTX65909 WB Image

WB analysis of various sample lysates using GTX65909 PRP8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PRP8 antibody used in IHC (Paraffin sections) (IHC-P). GTX65909

GTX65909 IHC-P Image

IHC-P analysis of rat brain tissue using GTX65909 PRP8 antibody.
Dilution : 1:100

Anti-PRP8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65909

GTX65909 ICC/IF Image

ICC/IF analysis of U-251 MG cells using GTX65909 PRP8 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399