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PRPF31 antibody

Anti-PRPF31 antibody used in IHC (Paraffin sections) (IHC-P). GTX117081
Anti-PRPF31 antibody used in IHC (Paraffin sections) (IHC-P). GTX117081
Anti-PRPF31 antibody used in Western Blot (WB). GTX117081

Cat. No. GTX117081

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PRPF31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Zebrafish, Bovine, Dog, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRPF31. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885412

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pre-mRNA processing factor 31 , NY-BR-99 , PRP31 , RP11 , SNRNP61

Cellular Localization

Nucleus speckle , Nucleus , Cajal body

Background

This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.

Database

Research Area

DATA IMAGES

Anti-PRPF31 antibody used in IHC (Paraffin sections) (IHC-P). GTX117081

GTX117081 IHC-P Image

PRPF31 antibody detects PRPF31 protein at nucleus speckles on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
PRPF31 antibody (GTX117081) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRPF31 antibody used in IHC (Paraffin sections) (IHC-P). GTX117081

GTX117081 IHC-P Image

PRPF31 antibody detects PRPF31 protein at nucleus speckles on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PRPF31 antibody (GTX117081) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRPF31 antibody used in Western Blot (WB). GTX117081

GTX117081 WB Image

PRPF31 antibody detects PRPF31 protein by western blot analysis.
A A549
B H1299
C HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
PRPF31 antibody (GTX117081) dilution: 1:1000

REFERENCE

Application Reference

Y Lv et al. J Biol Chem 2024; : 105772 The splicing factor Prpf31 is required for hematopoietic stem and progenitor cell expansion during zebrafish embryogenesis
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169