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PRPF39 antibody [N2C1], Internal

Overexpression
Anti-PRPF39 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104949
Anti-PRPF39 antibody [N2C1], Internal used in Western Blot (WB). GTX104949
Anti-PRPF39 antibody [N2C1], Internal used in Western Blot (WB). GTX104949
Anti-PRPF39 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104949

Cat. No. GTX104949

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PRPF39 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

Raji , human 3xFlag-PRPF39 transfected 293T cells

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRPF39. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037773

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pre-mRNA processing factor 39

Cellular Localization

Nucleus

Background

Involved in pre-mRNA splicing.

Database

Research Area

DATA IMAGES

Anti-PRPF39 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104949

GTX104949 IHC-P Image

PRPF39 antibody [N2C1], Internal detects PRPF39 protein at nucleus in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
PRPF39 antibody [N2C1], Internal (GTX104949) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PRPF39 antibody [N2C1], Internal used in Western Blot (WB). GTX104949

GTX104949 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX104949 diluted at 1:1000

Anti-PRPF39 antibody [N2C1], Internal used in Western Blot (WB). GTX104949

GTX104949 WB Image

Overexpression

PRPF39 antibody detects PRPF39 protein by western blot analysis. Non-transfected (-) and PRPF39-transfected (+, including Flag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRPF39 antibody (GTX104949) diluted by 1:5000.

Anti-PRPF39 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104949

GTX104949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PRPF39(GTX104949) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhou F et al. Cancer Discov 2023; 13 (2) : 332-347 A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid Leukemia.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169