GeneTex
United States (US)

PRPF39 antibody [N2C1], Internal

Overexpression
GTX104949_IHC_19011506_332.jpg
GTX104949_WB_19011506_137.jpg
GTX104949_40142_20151227_IHC-P_19011506_930.jpg
GTX104949_40142_WB_B_19011506_788.jpg

Cat No. GTX104949

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

Raji , 293T , human 3xFlag-PRPF39 transfected 293T

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRPF39. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pre-Mrna Processing Factor 39,Prpf39

Cellular Localization

Nucleus

Background

Involved in pre-mRNA splicing.

Database

DATA IMAGES
GTX104949_IHC_19011506_332.jpg

GTX104949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PRPF39(GTX104949) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104949_WB_19011506_137.jpg

GTX104949 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX104949 diluted at 1:1000

GTX104949_40142_20151227_IHC-P_19011506_930.jpg

GTX104949 IHC-P Image

PRPF39 antibody [N2C1], Internal detects PRPF39 protein at nucleus in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
PRPF39 antibody [N2C1], Internal (GTX104949) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104949_40142_WB_B_19011506_788.jpg

GTX104949 WB Image

Overexpression

PRPF39 antibody detects PRPF39 protein by western blot analysis. Non-transfected (-) and PRPF39-transfected (+, including Flag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRPF39 antibody (GTX104949) diluted by 1:5000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169