Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PRPS1 antibody (GTX102570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PRPS1(GTX102570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using PRPS1(GTX102570) antibody at 1:200 dilution.