GeneTex
United States (US)

PRPS1 antibody

GTX102639_39673_IHC_R_19011506_339.jpg
GTX102639_39673_WB_M_Kidney_19011506_346.jpg
GTX102639_39673_WB_R_Liver_19011506_946.jpg
GTX102639_WB_19011506_932.jpg
GTX102639_39673_IHC_M_19011506_563.jpg
GTX102639_39673_IHC_19011506_301.jpg

Cat No. GTX102639

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , rat liver , mouse kidney

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PRPS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoribosyl Pyrophosphate Synthetase 1,Arts,Cmtx5,Dfn2,Dfnx1,Ppribp,Prs-I,Prsi,Prps1

Background

This gene encodes an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Defects in this gene are a cause of phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5 and Arts Syndrome. [provided by RefSeq]

Database

DATA IMAGES
GTX102639_39673_IHC_R_19011506_339.jpg

GTX102639 IHC-P Image

PRPS1 antibody detects PRPS1 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PRPS1 antibody (GTX102639) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102639_39673_WB_M_Kidney_19011506_346.jpg

GTX102639 WB Image

Sample (50 ug of whole cell lysate)
A: mouse kidney
10% SDS PAGE
GTX102639 diluted at 1:3000

GTX102639_39673_WB_R_Liver_19011506_946.jpg

GTX102639 WB Image

Sample (50 ug of whole cell lysate)
A: Rat liver
10% SDS PAGE
GTX102639 diluted at 1:10000

GTX102639_WB_19011506_932.jpg

GTX102639 WB Image

Sample(30 μg of whole cell lysate)
A:293T
7.5% SDS PAGE
GTX102639 diluted at 1:1500

GTX102639_39673_IHC_M_19011506_563.jpg

GTX102639 IHC-P Image

PRPS1 antibody detects PRPS1 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
PRPS1 antibody (GTX102639) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102639_39673_IHC_19011506_301.jpg

GTX102639 IHC-P Image

PRPS1 antibody detects PRPS1 protein at cytosol on TOV-21G xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded TOV-21G xenograft.
PRPS1 antibody (GTX102639) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169