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PRPSAP2 antibody [N1C3]

Anti-PRPSAP2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107648
Anti-PRPSAP2 antibody [N1C3] used in Western Blot (WB). GTX107648
Anti-PRPSAP2 antibody [N1C3] used in Western Blot (WB). GTX107648
Anti-PRPSAP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107648
Anti-PRPSAP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107648

Cat. No. GTX107648

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Hamster
Package
100 μl ($399),
25 μl ($169)
See all PRPSAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Mouse liver , HeLaS3

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRPSAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951481

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoribosyl pyrophosphate synthetase associated protein 2 , PAP41

Background

The enzyme phosphoribosylpyrophosphate synthetase (PRS) catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRPSAP2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107648

GTX107648 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PRPSAP2(GTX107648) antibody at 1:500 dilution.

Anti-PRPSAP2 antibody [N1C3] used in Western Blot (WB). GTX107648

GTX107648 WB Image

Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX107648 diluted at 1:1000

Anti-PRPSAP2 antibody [N1C3] used in Western Blot (WB). GTX107648

GTX107648 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX107648 diluted at 1:1000

Anti-PRPSAP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107648

GTX107648 IHC-P Image

PRPSAP2 antibody [N1C3] detects PRPSAP2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PRPSAP2 antibody [N1C3] (GTX107648) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PRPSAP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107648

GTX107648 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PRPSAP2(GTX107648) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wu Y et al. Exp Cell Res 2019; 379 (1) : 30-47 ITGA6 and RPSA synergistically promote pancreatic cancer invasion and metastasis via PI3K and MAPK signaling pathways.
Application : ICC/IF
Reactivity : Hamster
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169