GeneTex
United States (US)

PRPSAP2 antibody [N1C3]

GTX107648_40009_IFA_19011506_686.jpg
GTX107648_40009_20150518_IHC_M_19011506_609.jpg
GTX107648_40009_IHC_19011506_593.jpg
GTX107648_WB_19011506_350.jpg
GTX107648_40009_WB_M_liver_19011507_636.jpg

Cat No. GTX107648

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRPSAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoribosyl Pyrophosphate Synthetase Associated Protein 2,Pap41,Prpsap2

Background

The enzyme phosphoribosylpyrophosphate synthetase (PRS) catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. [provided by RefSeq]

Database

DATA IMAGES
GTX107648_40009_IFA_19011506_686.jpg

GTX107648 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PRPSAP2(GTX107648) antibody at 1:500 dilution.

GTX107648_40009_20150518_IHC_M_19011506_609.jpg

GTX107648 IHC-P Image

PRPSAP2 antibody [N1C3] detects PRPSAP2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PRPSAP2 antibody [N1C3] (GTX107648) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107648_40009_IHC_19011506_593.jpg

GTX107648 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PRPSAP2(GTX107648) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107648_WB_19011506_350.jpg

GTX107648 WB Image

Sample (30μg whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX107648 diluted at 1:1000

GTX107648_40009_WB_M_liver_19011507_636.jpg

GTX107648 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX107648 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169