*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1:500-1:3000
ICC/IF
Assay dependent
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
PRRS virus
Immunogen
Recombinant protein encompassing a sequence within the center region of PRRS virus GP5 protein (PRRS virus (strain VR-2332)). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885883
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Porcine reproductive and respiratory syndrome virus GP5 envelope protein (ORF5) , Porcine reproductive and respiratory syndrome virus ORF5 , PRRSV GP5 envelope protein (ORF5) , PRRSV ORF5
Non-infected (–) and infected (+) Marc-145 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus GP5 protein antibody (GTX129062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129062 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus GP5 protein antibody (GTX129062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129062 WB Image
Non-infected (–) and infected (+) Marc-145 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus GP5 protein antibody (GTX129062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.