GeneTex
United States (US)

PRRS virus M protein antibody

GTX129063_42613_20170407_IFA_PRRSvirus_18121410_313.jpg
GTX129063_42613_20170818_WB_PRRSV_18121410_838.jpg
GTX129063_42613_20170426_IHC-P_PRRSV_18121410_988.jpg

Cat No. GTX129063

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity PRRS virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

North American (type II) genotypes PRRSV-infected Marc-145
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

PRRS virus

Immunogen

Recombinant protein encompassing a sequence within the center region of PRRS virus M protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PRRS virus Matrix antibody, PRRS virus ORF6 antibody, Porcine reproductive and respiratory syndrome virus Matrix antibody

Research Area

DATA IMAGES
GTX129063_42613_20170407_IFA_PRRSvirus_18121410_313.jpg

GTX129063 ICC/IF Image

Immunofluorescent analysis of PRRSV infected Marc-145 cells using PRRS virus M protein antibody . Sample: Marc-145 cells mock (left) and infected with North American (type II) genotypes PRRSV (right) were fixed in 4% formaldehyde at 4℃ for 10 min. Green: PRRS virus M protein stained by PRRS virus M protein antibody (GTX129063) diluted at 1:500.

GTX129063_42613_20170818_WB_PRRSV_18121410_838.jpg

GTX129063 WB Image

Non-infected (–) and infected (+) Marc-145 whole cell extracts (15 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus M protein antibody (GTX129063) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129063_42613_20170426_IHC-P_PRRSV_18121410_988.jpg

GTX129063 IHC-P Image

PRRS virus M protein antibody detects PRRSV at macrophages in PRRS virus-infected swine lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded PRRS virus-infected swine lymph node.
PRRS virus M protein antibody (GTX129063) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169