PRRS virus Nucleocapsid protein antibody [HL4257]

PRRS virus Nucleocapsid protein antibody [HL4257] detects PRRS virus Nucleocapsid protein (strain Lelystad) by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRRS virus Nucleocapsid protein (strain Lelystad) stained by PRRS virus Nucleocapsid protein antibody [HL4257] (GTX643493) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus Nucleocapsid protein antibody [HL4257] (GTX643493) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) Marc-145 whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus Nucleocapsid protein antibody [HL4257] (GTX643493) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.