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PRRS virus Nucleocapsid protein antibody

Anti-PRRS virus Nucleocapsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129270
Anti-PRRS virus Nucleocapsid protein antibody used in Western Blot (WB). GTX129270
Anti-PRRS virus Nucleocapsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129270

Cat No. GTX129270

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 PRRS virus
Package
100 μl ($379),
25 μl ($169)
See all PRRS virus Nucleocapsid protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

PRRSV-infected Marc-145 extracts

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

PRRS virus

Immunogen

Full length PRRS virus Nucleocapsid recombinant protein. (PRRS virus (strain US5))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885948

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Porcine reproductive and respiratory syndrome virus ORF7 , PRRSV ORF7

Research Area

DATA IMAGES

Anti-PRRS virus Nucleocapsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129270

GTX129270 ICC/IF Image

Immunofluorescent analysis of PRRSV infected Marc-145 cells using PRRS Virus Nucleocapsid protein antibody . Sample: Marc-145 cells mock (left) and infected with North American (type II) genotypes PRRSV (right) were fixed in 4% formaldehyde at 4ºC for 10 min. Green: PRRS Virus Nucleocapsid protein stained by PRRS Virus Nucleocapsid protein antibody (GTX129270) diluted at 1:1000.

Anti-PRRS virus Nucleocapsid protein antibody used in Western Blot (WB). GTX129270

GTX129270 WB Image

Non-infected (–) and infected (+) Marc-145 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS Virus Nucleocapsid protein antibody (GTX129270) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRRS virus Nucleocapsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129270

GTX129270 ICC/IF Image

PRRS virus Nucleocapsid protein antibody detects PRRS virus Nucleocapsid protein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRRS virus Nucleocapsid protein stained by PRRS virus Nucleocapsid protein antibody (GTX129270) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

Application Reference

Zhang L et al. J Virol 2022; : jvi0000522 Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway.
Application : WB
Takenouchi T et al. In Vitro Cell Dev Biol Anim 2021; 57 (1) : 10-16 Dexamethasone enhances CD163 expression in porcine IPKM immortalized macrophages.
Application : WB
Morozumi T et al. J Virol Methods 2021; 288 : 114026 Susceptibility of immortalized porcine kidney macrophages to porcine reproductive and respiratory syndrome virus-2 infection.
Application : ICC/IF
You X et al. Front Immunol 2020; 11 : 547144 Mir-331-3p Inhibits PRRSV-2 Replication and Lung Injury by Targeting PRRSV-2 ORF1b and Porcine TNF-α.
Application : WB
Chang X et al. FASEB J 2020; 34 (3) : 4497-4511 miR-382-5p promotes porcine reproductive and respiratory syndrome virus (PRRSV) replication by negatively regulating the induction of type I interferon.
Application : WB
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REVIEW

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Application Reference
Zhang L et al. J Virol 2022;:jvi0000522 Tumor Susceptibility Gene 101 (TSG101) Contributes to Virion Formation of Porcine Reproductive and Respiratory Syndrome Virus via Interaction with the Nucleocapsid (N) Protein along with the Early Secretory Pathway.
Application : WB
Takenouchi T et al. In Vitro Cell Dev Biol Anim 2021; 57 (1):10-16 Dexamethasone enhances CD163 expression in porcine IPKM immortalized macrophages.
Application : WB
Morozumi T et al. J Virol Methods 2021; 288:114026 Susceptibility of immortalized porcine kidney macrophages to porcine reproductive and respiratory syndrome virus-2 infection.
Application : ICC/IF
You X et al. Front Immunol 2020; 11:547144 Mir-331-3p Inhibits PRRSV-2 Replication and Lung Injury by Targeting PRRSV-2 ORF1b and Porcine TNF-α.
Application : WB
Chang X et al. FASEB J 2020; 34 (3):4497-4511 miR-382-5p promotes porcine reproductive and respiratory syndrome virus (PRRSV) replication by negatively regulating the induction of type I interferon.
Application : WB
Wang X et al. J Virol Methods 2019; 274:113727 A simple and efficient method for the generation of a porcine alveolar macrophage cell line for high-efficiency Porcine reproductive and respiratory syndrome virus 2 infection.
Application : WB, ICC/IF
Zhou L et al. Viruses. 2018; 10 (10):pii: E551. Genetic Characterization and Pathogenicity of a Novel Recombined Porcine Reproductive and Respiratory Syndrome Virus 2 among Nadc30-Like, Jxa1-Like, and Mlv-Like Strains.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169