GeneTex
United States (US)

PRRS virus Nucleocapsid protein antibody

GTX129270_42690_20170414_ICC_IF_PRRSvirus_18121410_769.jpg
GTX129270_42690_20170414_WB_PRRSvirus_18121410_249.jpg

Cat No. GTX129270

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity PRRS virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

PRRSV-infected Marc-145 whole cell extracts , PRRSV-infected Marc-145 whole cell extracts
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

PRRS virus

Immunogen

Full length PRRS virus Nucleocapsid recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX129270_42690_20170414_ICC_IF_PRRSvirus_18121410_769.jpg

GTX129270 ICC/IF Image

Immunofluorescent analysis of PRRSV infected Marc-145 cells using PRRS Virus Nucleocapsid protein antibody . Sample: Marc-145 cells mock (left) and infected with North American (type II) genotypes PRRSV (right) were fixed in 4% formaldehyde at 4℃ for 10 min. Green: PRRS Virus Nucleocapsid protein stained by PRRS Virus Nucleocapsid protein antibody (GTX129270) diluted at 1:1000.

GTX129270_42690_20170414_WB_PRRSvirus_18121410_249.jpg

GTX129270 WB Image

Non-infected (–) and infected (+) Marc-145 whole cell extracts (15 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS Virus Nucleocapsid protein antibody (GTX129270) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169