GeneTex
United States (US)

PRX I antibody [N1C2]

GTX101705_WB_M_19011506_358.jpg
GTX101705_WB_19011506_402.jpg
GTX101705_IFA_19011506_229.jpg
GTX101705_39577_IHC_M_19011506_468.jpg

Cat No. GTX101705

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10.00mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRX I. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Peroxiredoxin 1,Msp23,Nkef-A,Nkefa,Pag,Paga,Pagb,Prx1,Prxi,Tdpx2,Prdx1

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101705_WB_M_19011506_358.jpg

GTX101705 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 12% SDS PAGE
GTX101705 diluted at 1:1000

GTX101705_WB_19011506_402.jpg

GTX101705 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX101705 diluted at 1:1000

GTX101705_IFA_19011506_229.jpg

GTX101705 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PRX I(GTX101705) antibody at 1:200 dilution.

GTX101705_39577_IHC_M_19011506_468.jpg

GTX101705 IHC-P Image

PRX I antibody [N1C2] detects PRX I protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PRX I antibody [N1C2] (GTX101705) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169