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PRX I antibody [N1C2]

KO/KD Orthogonal
Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705
Anti-PRX I antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101705
Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705
Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705
Anti-PRX I antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101705
Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705
Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705
Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705

Cat. No. GTX101705

Reference 9
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PRX I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , NIH-3T3

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRX I. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1241175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 1 , MSP23 , NKEF-A , NKEFA , PAG , PAGA , PAGB , PRX1 , PRXI , TDPX2

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705

GTX101705 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] (GTX101705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRX I antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101705

GTX101705 ICC/IF Image

PRX I antibody [N1C2] detects PRX I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PRX I stained by PRX I antibody [N1C2] (GTX101705) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705

GTX101705 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C2] (GTX101705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705

GTX101705 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX101705 diluted at 1:1000

Anti-PRX I antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101705

GTX101705 IHC-P Image

PRX I antibody [N1C2] detects PRX I protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PRX I antibody [N1C2] (GTX101705) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705

GTX101705 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 12% SDS PAGE
GTX101705 diluted at 1:1000

Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705

GTX101705 WB Image

Orthogonal

The data was published in the journal Proteome Sci in 2014. PMID: 24405781

Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705

GTX101705 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770

REFERENCE

Application Reference

Peng SY et al. Antioxidants (Basel) 2021; 10 (7) Pomegranate Extract (POMx) Induces Mitochondrial Dysfunction and Apoptosis of Oral Cancer Cells.
Application : WB
Reactivity : Human
Jadhao M et al. Antioxidants (Basel) 2021; 10 (6) The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS.
Application : WB
Reactivity : Human
Liu W et al. Autophagy 2021; : 44219
Application : WB, ICC/IF
Reactivity : Mouse
Liu W et al. Oxid Med Cell Longev 2020; 2020 : 5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
Wangta Liu et al. Oxidative Medicine and Cellular Longevity 2020; 2020 : Article ID 5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

PRX I antibody [N1C2] Cat. No. GTX101705

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-PRX I antibody [N1C2] used in Western Blot (WB). GTX101705
Date : Anonymous submitted on 05-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10 μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Peng SY et al. Antioxidants (Basel) 2021; 10 (7) Pomegranate Extract (POMx) Induces Mitochondrial Dysfunction and Apoptosis of Oral Cancer Cells.
Application : WB
Reactivity : Human
Jadhao M et al. Antioxidants (Basel) 2021; 10 (6) The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS.
Application : WB
Reactivity : Human
Liu W et al. Autophagy 2021;:44219
Application : WB, ICC/IF
Reactivity : Mouse
Liu W et al. Oxid Med Cell Longev 2020; 2020:5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
Wangta Liu et al. Oxidative Medicine and Cellular Longevity 2020; 2020:Article ID 5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
Application : WB
Reactivity : Human
Chou HC et al. Proteome Sci 2014; 12 (1):2 Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Chou HC et al. Arch Biochem Biophys 2014; 543:15-22 5-Methoxytryptophan-dependent protection of cardiomyocytes from heart ischemia reperfusion injury.
Application : WB
Reactivity : Rat
Ren P et al. Oncol Rep 2013; 30 (5):2297-303 Peroxiredoxin 1 is a tumor-associated antigen in esophageal squamous cell carcinoma.
Application : WB
Reactivity : Human
Fan CY et al. Electrophoresis 2013; 34 (24):3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169