PRX I antibody [N1C3]

Name: PRX I antibody [N1C3]
Brand: GeneTex
SKU: GTX113793
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-PRX I antibody [N1C3] used in Western Blot (WB). GTX113793

Anti-PRX I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113793

Cat. No. GTX113793

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all PRX I products

View product citations for antibody GTX113793 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRX I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037651

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 1 , MSP23 , NKEF-A , NKEFA , PAG , PAGA , PAGB , PRX1 , PRXI , TDPX2

Cellular Localization

Cytoplasm , Melanosome

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

Gene ID: 5052 PRDX1
UniProt: Q06830 PRDX1

Research Area

DATA IMAGES

GTX113793 WB Image

Wild-type (WT) and PRDX1 knockout (KO) U2OS cell extracts (10 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C3] (GTX113793) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.

GTX113793 WB Image

Wild-type (WT) and PRDX1 knockout (KO) U2OS cell extracts (10 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C3] (GTX113793) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.<br><I>Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.</I>

GTX113793 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113793 diluted at 1:1000

GTX113793 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using PRX I(GTX113793) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113793 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX I antibody [N1C3] (GTX113793) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

R Ayoubi et al. Elife 2023; 12 : RP91645 Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications

Xu YM et al. Toxicol Res (Camb) 2019; 8 (6) : 994-1001 Cadmium telluride quantum dot-exposed human bronchial epithelial cells: a further study of the cellular response by proteomics.

Chen DJ et al. Proteomics. 2015; Proteomic analysis of secreted proteins by human bronchial epithelial cells in response to cadmium toxicity.[Epub ahead of print]

Application : WB

Reactivity : Human

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