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PRX I antibody [N1C3]

Anti-PRX I antibody [N1C3] used in Western Blot (WB). GTX113793
Anti-PRX I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113793
Anti-PRX I antibody [N1C3] used in Western Blot (WB). GTX113793

Cat No. GTX113793

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRX I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037651

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peroxiredoxin 1 , MSP23 , NKEF-A , NKEFA , PAG , PAGA , PAGB , PRX1 , PRXI , TDPX2

Cellular Localization

Cytoplasm , Melanosome

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRX I antibody [N1C3] used in Western Blot (WB). GTX113793

GTX113793 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113793 diluted at 1:1000

Anti-PRX I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX113793

GTX113793 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using PRX I(GTX113793) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRX I antibody [N1C3] used in Western Blot (WB). GTX113793

GTX113793 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2 (GTX27900)
12% SDS PAGE
GTX113793 diluted at 1:1000

REFERENCE

REVIEW

PRX I antibody [N1C3] Cat No. GTX113793

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 10 μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169