GeneTex
United States (US)

PRX I antibody

GTX15571_1497_WB_19011519_764.jpg
GTX15571_266_ICC-IF_19011519_474.jpg
GTX15571_265_ICC-IF_19011519_959.jpg
GTX15571_1498_WB_19011519_903.jpg

Cat No. GTX15571

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000 - 1:5,000
ICC/IF 1:250
IHC Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific for Prx 1 and shows no cross-reactivity with other Prx isoforms.
PROPERTIES

Form

Liquid

Buffer

Serum, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues L(103) V S D P K R T I A Q D(114) of human Peroxiredoxin 1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Peroxiredoxin 1,Msp23,Nkef-A,Nkefa,Pag,Paga,Pagb,Prx1,Prxi,Tdpx2,Prdx1

Cellular Localization

Cytoplasm,Melanosome

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES
GTX15571_1497_WB_19011519_764.jpg

GTX15571 WB Image

WB analysis of 25 ug of various whole cell lysates using GTX15571 PRX I antibody.
Dilution : 1:1000

GTX15571_266_ICC-IF_19011519_474.jpg

GTX15571 ICC/IF Image

ICC/IF analysis of TSU-Pr1 cells using GTX15571 PRX I antibody.

GTX15571_265_ICC-IF_19011519_959.jpg

GTX15571 ICC/IF Image

ICC/IF analysis of T-47D cells using GTX15571 PRX I antibody. Panel e shows the no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

GTX15571_1498_WB_19011519_903.jpg

GTX15571 WB Image

WB analysis of whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Raji (Lane 2) and Ramos (Lane 3) using GTX15571 PRX I antibody.
Dilution : 0.5 µg/ml

REFERENCE
Package List Price ($)
$ 339