PRX III antibody [N1C3]

Name: PRX III antibody [N1C3]
Brand: GeneTex
SKU: GTX112004
Price: 419.00 USD
Rating: 5 (1 reviews)

Anti-PRX III antibody [N1C3] used in Western Blot (WB). GTX112004

Anti-PRX III antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112004

Anti-PRX III antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX112004

Cat. No. GTX112004

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($419), 25 μl ($169)

See all PRX III products

Datasheet
Document

View product citations for antibody GTX112004 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRX III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951376

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 3 , AOP-1 , AOP1 , HBC189 , MER5 , PRO1748 , SP-22 , prx-III

Cellular Localization

Mitochondrion

Background

This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 10935 PRDX3
UniProt: P30048 PRDX3

Research Area

DATA IMAGES

GTX112004 WB Image

Wild-type (WT) and PRX III knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX III antibody [N1C3] (GTX112004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112004 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112004 diluted at 1:1000

GTX112004 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
C: Molt-4 (GTX27912)
D: Raji
12% SDS PAGE
GTX112004 diluted at 1:1000

GTX112004 ICC/IF Image

Peroxiredoxin 3 antibody [N1C3] detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3] (GTX112004) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX112004 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Peroxiredoxin 3(GTX112004) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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