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PRX IV antibody

Anti-PRX IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108256
Anti-PRX IV antibody used in Western Blot (WB). GTX108256
Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108256
Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108256
Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108256

Cat. No. GTX108256

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRX IV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRX IV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951379

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 4 , AOE37-2 , AOE372 , HEL-S-97n , PRX-4

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRX IV antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108256

GTX108256 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using peroxiredoxin 4(GTX108256) antibody at 1:500 dilution.

Anti-PRX IV antibody used in Western Blot (WB). GTX108256

GTX108256 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX IV antibody (GTX108256) diluted at 1:500.

Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108256

GTX108256 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PRX IV(GTX108256) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108256

GTX108256 IHC-P Image

peroxiredoxin 4 antibody detects peroxiredoxin 4 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
peroxiredoxin 4 antibody (GTX108256) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX108256

GTX108256 IHC-P Image

peroxiredoxin 4 antibody detects peroxiredoxin 4 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
peroxiredoxin 4 antibody (GTX108256) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169