GeneTex
United States (US)

PRX IV antibody

GTX108256_39778_IHC_19011507_900.jpg
GTX108256_39778_20160728_WB_19011507_261.jpg
GTX108256_39778_20141231_IHC_M_19011507_157.jpg
GTX108256_39778_IFA_19011507_385.jpg
GTX108256_39778_20141231_IHC_M_2_19011507_148.jpg

Cat No. GTX108256

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human peroxiredoxin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Peroxiredoxin 4,Aoe37-2,Aoe372,Hel-S-97N,Prx-4,Prdx4

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108256_39778_IHC_19011507_900.jpg

GTX108256 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PRX IV(GTX108256) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108256_39778_20160728_WB_19011507_261.jpg

GTX108256 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX IV antibody (GTX108256) diluted at 1:500.

GTX108256_39778_20141231_IHC_M_19011507_157.jpg

GTX108256 IHC-P Image

peroxiredoxin 4 antibody detects peroxiredoxin 4 protein at cytoplasm on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
peroxiredoxin 4 antibody (GTX108256) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108256_39778_IFA_19011507_385.jpg

GTX108256 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using peroxiredoxin 4(GTX108256) antibody at 1:500 dilution.

GTX108256_39778_20141231_IHC_M_2_19011507_148.jpg

GTX108256 IHC-P Image

peroxiredoxin 4 antibody detects peroxiredoxin 4 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
peroxiredoxin 4 antibody (GTX108256) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169