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PRX IV antibody

Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX116414
Anti-PRX IV antibody used in Western Blot (WB). GTX116414
Anti-PRX IV antibody used in Western Blot (WB). GTX116414

Cat. No. GTX116414

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRX IV products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Mouse brain , NT2D1

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRX IV. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617688

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 4 , AOE37-2 , AOE372 , HEL-S-97n , PRX-4

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRX IV antibody used in IHC (Paraffin sections) (IHC-P). GTX116414

GTX116414 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PRX IV(GTX116414) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PRX IV antibody used in Western Blot (WB). GTX116414

GTX116414 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
12% SDS PAGE
GTX116414 diluted at 1:1000

Anti-PRX IV antibody used in Western Blot (WB). GTX116414

GTX116414 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX116414 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169