GeneTex
United States (US)

PRX IV antibody

GTX15574_269_ICC-IF_19011519_175.jpg
GTX15574_1499_WB_19011519_213.jpg
GTX15574_268_ICC-IF_19011519_952.jpg

Cat No. GTX15574

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:250
IHC Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific for Prx 4 and shows no cross-reactivity with other Prx isoforms.
PROPERTIES

Form

Liquid

Buffer

Serum, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified human recombinant Peroxiredoxin 4.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Peroxiredoxin 4,Aoe37-2,Aoe372,Hel-S-97N,Prx-4,Prdx4

Cellular Localization

Cytoplasm,Endoplasmic reticulum

Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15574_269_ICC-IF_19011519_175.jpg

GTX15574 ICC/IF Image

ICC/IF analysis of TSU-Pr1 cells using GTX15574 PRX IV antibody.

GTX15574_1499_WB_19011519_213.jpg

GTX15574 WB Image

WB analysis of membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), DU-145 (Lane 2), PC-3 (Lane 3), LNCaP (Lane 4), MDA-MB-231 (Lane 5), Hep G2 (Lane 6), A549 (Lane 7) and U-937 (Lane 8) using GTX15574 PRX IV antibody.
Dilution : 1:5000

GTX15574_268_ICC-IF_19011519_952.jpg

GTX15574 ICC/IF Image

ICC/IF analysis of LNCaP cells using GTX15574 PRX IV antibody. Panel e shows the no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE
Package List Price ($)
$ 319