ICC/IF analysis of TSU-Pr1 cells using GTX15574 PRX IV antibody.

ICC/IF analysis of LNCaP cells using GTX15574 PRX IV antibody. Panel e shows the no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

WB analysis of membrane enriched extracts (30 μg lysate) of MCF7 (Lane 1), DU-145 (Lane 2), PC-3 (Lane 3), LNCaP (Lane 4), MDA-MB-231 (Lane 5), Hep G2 (Lane 6), A549 (Lane 7) and U-937 (Lane 8) using GTX15574 PRX IV antibody.
Dilution : 1:5000