This antibody is specific for Prx 4 and shows no cross-reactivity with other Prx isoforms.
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified human recombinant Peroxiredoxin 4.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_367803
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]
WB analysis of membrane enriched extracts (30 μg lysate) of MCF7 (Lane 1), DU-145 (Lane 2), PC-3 (Lane 3), LNCaP (Lane 4), MDA-MB-231 (Lane 5), Hep G2 (Lane 6), A549 (Lane 7) and U-937 (Lane 8) using GTX15574 PRX IV antibody. Dilution : 1:5000
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GTX15574 ICC/IF Image
ICC/IF analysis of LNCaP cells using GTX15574 PRX IV antibody. Panel e shows the no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
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GTX15574 ICC/IF Image
ICC/IF analysis of TSU-Pr1 cells using GTX15574 PRX IV antibody.
