APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
PSA from human sperm plasma
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
APS , KLK2A1 , KLK3 , PSA , hK3 , kallikrein related peptidase 3 , Prostate specific antigen
Cellular Localization
Secreted
Background
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-PSA antibody [SPM352] used in IHC (Paraffin sections) (IHC-P). GTX34989](/templates/default/js/lightbox/images/loading.gif "Anti-PSA antibody [SPM352] used in IHC (Paraffin sections) (IHC-P). GTX34989")
|
GTX34989 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX34989 PSA antibody [SPM352].
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REFERENCE
There are currently no references for PSA antibody [SPM352] (GTX34989). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSA antibody [SPM352] (GTX34989). Be the first to share your experience with this product.
![Anti-PSA antibody [SPM352] used in IHC (Paraffin sections) (IHC-P). GTX34989](/upload/website/prouct_img/small/GTX34989/GTX34989_20200115_IHC-P_1046_20011518_555.jpg "Anti-PSA antibody [SPM352] used in IHC (Paraffin sections) (IHC-P). GTX34989")
![Anti-PSA antibody [SPM352] used in IHC (Paraffin sections) (IHC-P). GTX34989](/upload/website/prouct_img/normal/GTX34989/GTX34989_20200115_IHC-P_1046_20011518_555.jpg "Anti-PSA antibody [SPM352] used in IHC (Paraffin sections) (IHC-P). GTX34989")