PSAP antibody [4D5F4]

Cat. No. GTX60673

Host	Mouse
Clonality	Monoclonal
Clone Name	4D5F4
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX60673 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/50-1/500
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PSAP (AA: 325-524 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prosaposin , GLBA , SAP1

Cellular Localization

Lysosome

Background

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5660 PSAP
UniProt: P07602 PSAP

Research Area

DATA IMAGES

GTX60673 WB Image

WB analysis of HEK293 (1) and PSAP (AA: 325-524)-hIgGFc transfected HEK293 (2) cell lysate using GTX60673 PSAP antibody [4D5F4].

GTX60673 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX60673 PSAP antibody [4D5F4].

GTX60673 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60673 PSAP antibody [4D5F4].
Green : PSAP
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60673 ELISA Image

ELISA analysis of antigen using GTX60673 PSAP antibody [4D5F4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60673 FACS Image

FACS analysis of HeLa cells using GTX60673 PSAP antibody [4D5F4].
Green : PSAP
Purple : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty