GeneTex
United States (US)

PSAP antibody [N1N3]

KO/KD
GTX101064_40142_20181109_WB_KO_watermark_19011519_646.jpg
GTX101064_40142_20170518_WB_19011506_236.jpg
GTX101064_40142_20170518_WB_M_R_19011506_278.jpg
GTX101064_IHC_19011506_654.jpg

Cat No. GTX101064

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prosaposin,Glba,Sap1,Psap

Cellular Localization

Lysosome

Background

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101064_40142_20181109_WB_KO_watermark_19011519_646.jpg

GTX101064 WB Image

KO/KD

Wild-type (WT) and PSAP knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] (GTX101064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101064_40142_20170518_WB_19011506_236.jpg

GTX101064 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] (GTX101064) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101064_40142_20170518_WB_M_R_19011506_278.jpg

GTX101064 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] (GTX101064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101064_IHC_19011506_654.jpg

GTX101064 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PSAP(GTX101064) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169