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PSAP antibody [N1N3]

KO/KD
Anti-PSAP antibody [N1N3] used in Western Blot (WB). GTX101064
Anti-PSAP antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101064
Anti-PSAP antibody [N1N3] used in Western Blot (WB). GTX101064
Anti-PSAP antibody [N1N3] used in Western Blot (WB). GTX101064
Anti-PSAP antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101064

Cat. No. GTX101064

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PSAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

mouse brain , rat brain , HeLa

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037779

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prosaposin , GLBA , SAP1

Cellular Localization

Lysosome

Background

This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSAP antibody [N1N3] used in Western Blot (WB). GTX101064

GTX101064 WB Image

KO/KD

Wild-type (WT) and PSAP knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] (GTX101064) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PSAP antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101064

GTX101064 IHC-P Image

PSAP antibody [N1N3] detects PSAP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
PSAP stained by PSAP antibody [N1N3] (GTX101064) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PSAP antibody [N1N3] used in Western Blot (WB). GTX101064

GTX101064 WB Image

Various extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] (GTX101064) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PSAP antibody [N1N3] used in Western Blot (WB). GTX101064

GTX101064 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAP antibody [N1N3] (GTX101064) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PSAP antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101064

GTX101064 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PSAP(GTX101064) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

A Fuyuki et al. Tissue Cell 2024; 88 : 102367 Selective prosaposin expression in Langerhans islets of the mouse pancreas
Application : WB
Fuyuki A et al. J Vet Med Sci 2023; 85 (3) : 266-270 Expression of prosaposin and its G protein-coupled receptor (GPR) 37 in mouse cochlear and vestibular nuclei.
Application : IHC-Fr
Reactivity : Mouse
Chiu CC et al. Mol Neurobiol 2018; (Epub) PARK14 (D331Y) PLA2G6 Causes Early-Onset Degeneration of Substantia Nigra Dopaminergic Neurons by Inducing Mitochondrial Dysfunction, ER Stress, Mitophagy Impairment and Transcriptional Dysregulation in a Knockin Mouse Model.
Application : WB
Reactivity : Mouse
Marceau C.D. et al. Nature 2016; 535 (7610) : 159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB
Reactivity : Human
Lai MC et al. PLoS One 2016; 11 (4) : e0153627 Hypoxia Induces Autophagy through Translational Up-Regulation of Lysosomal Proteins in Human Colon Cancer Cells.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169