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PSAT1 antibody [HL2663]

Anti-PSAT1 antibody [HL2663] used in Western Blot (WB). GTX639327
Anti-PSAT1 antibody [HL2663] used in IHC (Paraffin sections) (IHC-P). GTX639327
Anti-PSAT1 antibody [HL2663] used in Western Blot (WB). GTX639327
Anti-PSAT1 antibody [HL2663] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639327
Anti-PSAT1 antibody [HL2663] used in Western Blot (WB). GTX639327

Cat. No. GTX639327

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2663

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human PSAT1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoserine aminotransferase 1 , EPIP , NLS2 , PSA , PSAT , PSATD

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-PSAT1 antibody [HL2663] used in Western Blot (WB). GTX639327

GTX639327 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [HL2663] (GTX639327) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PSAT1 antibody [HL2663] used in IHC (Paraffin sections) (IHC-P). GTX639327

GTX639327 IHC-P Image

PSAT1 antibody [HL2663] detects PSAT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PSAT1 stained by PSAT1 antibody [HL2663] (GTX639327) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PSAT1 antibody [HL2663] used in Western Blot (WB). GTX639327

GTX639327 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [HL2663] (GTX639327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PSAT1 antibody [HL2663] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639327

GTX639327 ICC/IF Image

PSAT1 antibody [HL2663] detects PSAT1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PSAT1 stained by PSAT1 antibody [HL2663] (GTX639327) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-PSAT1 antibody [HL2663] used in Western Blot (WB). GTX639327

GTX639327 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [HL2663] (GTX639327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations