APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , A549 , H1299 , HCT116 , 293T
Predict Reactivity
Rat, Rabbit, Bovine, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.33 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSAT1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951485
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphoserine aminotransferase 1 , EPIP , NLS2 , PSA , PSAT , PSATD
Background
The protein encoded by this gene is likely a phosphoserine aminotransferase, based on similarity to proteins in mouse, rabbit, and Drosophila. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576](/templates/default/js/lightbox/images/loading.gif "Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576")
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GTX110576 WB Image
PSAT1 antibody [N1C3] detects PSAT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3] (GTX110576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110576 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3] (GTX110576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110576 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PSAT1(GTX110576) antibody at 1:200 dilution.
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GTX110576 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PSAT1(GTX110576) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110576 WB Image
Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX110576 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for PSAT1 antibody [N1C3] (GTX110576). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSAT1 antibody [N1C3] (GTX110576). Be the first to share your experience with this product.
![Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576](/upload/website/prouct_img/small/GTX110576/GTX110576_42242_20151008_WB_w_23060500_740.webp "Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576](/upload/website/prouct_img/small/GTX110576/GTX110576_42242_20161124_WB_shRNA_watermark_w_23060500_748.webp "Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110576](/upload/website/prouct_img/small/GTX110576/GTX110576_IFA_w_23060500_543.webp "Anti-PSAT1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110576](/upload/website/prouct_img/small/GTX110576/GTX110576_40037_IHC_w_23060500_620.webp "Anti-PSAT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576](/upload/website/prouct_img/small/GTX110576/GTX110576_40037_WB_M_brain_w_23060500_627.webp "Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576](/upload/website/prouct_img/normal/GTX110576/GTX110576_42242_20151008_WB_w_23060500_740.webp "Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576](/upload/website/prouct_img/normal/GTX110576/GTX110576_42242_20161124_WB_shRNA_watermark_w_23060500_748.webp "Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110576](/upload/website/prouct_img/normal/GTX110576/GTX110576_IFA_w_23060500_543.webp "Anti-PSAT1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110576](/upload/website/prouct_img/normal/GTX110576/GTX110576_40037_IHC_w_23060500_620.webp "Anti-PSAT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110576")
![Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576](/upload/website/prouct_img/normal/GTX110576/GTX110576_40037_WB_M_brain_w_23060500_627.webp "Anti-PSAT1 antibody [N1C3] used in Western Blot (WB). GTX110576")