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PSD95 antibody [GT1234]

Anti-PSD95 antibody [GT1234] used in Western Blot (WB). GTX634291
Anti-PSD95 antibody [GT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634291
Anti-PSD95 antibody [GT1234] used in Western Blot (WB). GTX634291

Cat No. GTX634291

Host Mouse
Clonality Monoclonal
Clone Name GT1234
Isotype IgG2b
Application WB, ICC/IF
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

rat brain , mouse brain

Predict Reactivity

Human, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse PSD95. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

discs large MAGUK scaffold protein 4,Dlgh4,PSD-95,PSD95,SAP90,SAP90A

Cellular Localization

Membrane , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

Database

Research Area

DATA IMAGES
Anti-PSD95 antibody [GT1234] used in Western Blot (WB). GTX634291

GTX634291 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody [GT1234] (GTX634291) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PSD95 antibody [GT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634291

GTX634291 ICC/IF Image

PSD95 antibody [GT1234] detects PSD95 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSD95 stained by PSD95 antibody [GT1234] (GTX634291) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PSD95 antibody [GT1234] used in Western Blot (WB). GTX634291

GTX634291 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody [GT1234] (GTX634291) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169