Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody [HL3165] (GTX640675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data are based on Human Protein Atlas program.

PSD95 antibody [HL3165] detects PSD95 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse eye.
Green: PSD95 stained by PSD95 antibody [HL3165] (GTX640675) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PSD95 antibody [HL3165] detects PSD95 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: PSD95 stained by PSD95 antibody [HL3165] (GTX640675) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PSD95 antibody [HL3165] detects PSD95 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: PSD95 stained by PSD95 antibody [HL3165] (GTX640675) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody [HL3165] (GTX640675) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody [HL3165] (GTX640675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.