GeneTex
United States (US)

PSD95 antibody

GTX133091_42508_20160921_IHC-Fr_18121410_748.jpg
GTX133091_42508_20160811_WB_M_eye_18121410_389.jpg
GTX133091_42508_20170719_IFA_R_18121410_369.jpg
GTX133091_42508_20160616_WB_M_R_18121410_289.jpg
GTX133091_42508_20170306_IHC-P_M_18121410_516.jpg

Cat No. GTX133091

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

mouse eye , mouse brain│mouse fetal brain│rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse PSD95. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dlg4 antibody , Dlgh4 antibody , PSD95 antibody , PSD95 antibody , SAP90 antibody , SAP90A antibody , discs, large homolog 4 (Drosophila) antibody

Cellular Localization

Membrane , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.

Database

Research Area

DATA IMAGES
GTX133091_42508_20160921_IHC-Fr_18121410_748.jpg

GTX133091 IHC-Fr Image

PSD95 antibody detects PSD95 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133091_42508_20160811_WB_M_eye_18121410_389.jpg

GTX133091 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody (GTX133091) diluted at 1:1000.

GTX133091_42508_20170719_IFA_R_18121410_369.jpg

GTX133091 ICC/IF Image

PSD95 antibody detects PSD95 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133091_42508_20160616_WB_M_R_18121410_289.jpg

GTX133091 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PSD95 antibody (GTX133091) diluted at 1:1000.

GTX133091_42508_20170306_IHC-P_M_18121410_516.jpg

GTX133091 IHC-P Image

PSD95 antibody detects PSD95 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: PSD95 protein stained by PSD95 antibody (GTX133091) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169