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PSKH1 antibody

Anti-PSKH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103525
Anti-PSKH1 antibody used in Western Blot (WB). GTX103525
Anti-PSKH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103525

Cat No. GTX103525

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLaS3 , HepG2

Predict Reactivity

Mouse, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSKH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

protein serine kinase H1

Cellular Localization

Golgi apparatus , Centrosome , Nucleus speckle , Endoplasmic reticulum membrane; Lipid-anchor , Cell membrane; Lipid-anchor , Cytoplasm

Background

May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.

Database

Research Area

DATA IMAGES
Anti-PSKH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103525

GTX103525 IHC-P Image

PSKH1 antibody detects PSKH1 protein at cytooplasm and membrane on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
PSKH1 antibody (GTX103525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSKH1 antibody used in Western Blot (WB). GTX103525

GTX103525 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX103525 diluted at 1:1000

Anti-PSKH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103525

GTX103525 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PSKH1(GTX103525) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169