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PSKH1 antibody

Anti-PSKH1 antibody used in Western Blot (WB). GTX103525
Anti-PSKH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103525
Anti-PSKH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103525

Cat. No. GTX103525

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PSKH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLaS3 , HepG2

Predict Reactivity

Mouse, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSKH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein serine kinase H1

Cellular Localization

Golgi apparatus , Centrosome , Nucleus speckle , Endoplasmic reticulum membrane; Lipid-anchor , Cell membrane; Lipid-anchor , Cytoplasm

Background

May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.

Database

Research Area

DATA IMAGES

Anti-PSKH1 antibody used in Western Blot (WB). GTX103525

GTX103525 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX103525 diluted at 1:1000

Anti-PSKH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103525

GTX103525 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PSKH1(GTX103525) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-PSKH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103525

GTX103525 IHC-P Image

PSKH1 antibody detects PSKH1 protein at cytooplasm and membrane on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
PSKH1 antibody (GTX103525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169