APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSKH1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885304
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein serine kinase H1
Cellular Localization
Golgi apparatus , Centrosome , Nucleus speckle , Endoplasmic reticulum membrane; Lipid-anchor , Cell membrane; Lipid-anchor , Cytoplasm
Background
May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.
Database
Research Area
DATA IMAGES
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GTX111473 IHC-P Image
PSKH1 antibody detects PSKH1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PSKH1 antibody (GTX111473) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111473 ICC/IF Image
PSKH1 antibody detects PSKH1 protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSKH1 protein stained by PSKH1 antibody (GTX111473) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX111473 WB Image
PSKH1 antibody detects PSKH1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSKH1 antibody (GTX111473) diluted by 1:1000.
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REFERENCE
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REVIEW
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