GeneTex
United States (US)

PSMA antibody [Y/PSMA1]

Cat No. GTX39345

Host Mouse
Clonality Monoclonal
Clone Name Y/PSMA1
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Specificity/Sensitivity

Prostate specific membrane antigen, extracellular domain. Reacts with human prostate carcinoma tissues andPSMA derived synthetic peptides. Does not react against normal human prostate tissues or to benignprostatic hyperplasia (BPH) tissues. Reactive to PSMA expressed LNCap cell lines.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH 7.0

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Crude membrane protein preparation from pooled human prostate adenocarcinoma tissues.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Folate Hydrolase 1,Fgcp,Folh,Gcp2,Gcpii,Naalad1,Naaladase,Psm,Psma,Mgcp,Folh1

Background

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

Database

Research Area

Package List Price ($)
$ 289