Application Note
For ELISA: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
LNCaP cell line
Product Note
Minimal or no cross-reactivity to benign prostate hyperplasia (BPH) or to normal prostatic tissue.
Form
Liquid
Buffer
0.01M PBS pH7.0
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Crude membrane protein preparation from pooled prostate malignant carcinoma from China.
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_423409
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
folate hydrolase 1 , FGCP , FOLH , GCP2 , GCPII , NAALAD1 , NAALAdase , PSM , PSMA , mGCP
Cellular Localization
Cell membrane
Background
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]
Database
Research Area