GeneTex
United States (US)

PSMA4 antibody, Internal

GTX88053_1090_WB_18122102_656.jpg
GTX88053_1091_WB_18122102_436.jpg

Cat No. GTX88053

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88053-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_002780.1; NP_001096138.1). Reported variants represent identical protein: NP_002780.1, NP_001096137.1

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-SDANVLTNELR, from the internal region of the protein sequence according to NP_002780.1; NP_001096138.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PSC9 Antibody , HC9 Antibody , HST17706 Antibody

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88053_1090_WB_18122102_656.jpg

GTX88053 WB Image

WB analysis of NIH-3T3 (A), mouse spleen (B), rat spleen (C)) lysates using GTX88053 PSMA4 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88053_1091_WB_18122102_436.jpg

GTX88053 WB Image

WB analysis of K562 cell lysate using GTX88053 PSMA4 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer