GeneTex
United States (US)

PSMA5 antibody

GTX103845_39918_IHC_19011506_194.jpg
GTX103845_39918_20151227_IHC-P_19011506_269.jpg
GTX103845_39918_WB_19011506_844.jpg
GTX103845_IFA_19011506_255.jpg
GTX103845_39918_WB_M_brain_19011506_307.jpg

Cat No. GTX103845

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMA5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proteasome Subunit Alpha 5,Psc5,Zeta,Psma5

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103845_39918_IHC_19011506_194.jpg

GTX103845 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Proteasome 20S alpha 5(GTX103845) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103845_39918_20151227_IHC-P_19011506_269.jpg

GTX103845 IHC-P Image

PSMA5 antibody detects PSMA5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
PSMA5 antibody (GTX103845) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103845_39918_WB_19011506_844.jpg

GTX103845 WB Image

PSMA5 antibody detects PSMA5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA5 antibody (GTX103845) diluted by 1:1000.

GTX103845_IFA_19011506_255.jpg

GTX103845 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Proteasome 20S alpha5(GTX103845) antibody at 1:200 dilution.

GTX103845_39918_WB_M_brain_19011506_307.jpg

GTX103845 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX103845 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169