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PSMA5 antibody

Anti-PSMA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11437
Anti-PSMA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11437
Anti-PSMA5 antibody used in Western Blot (WB). GTX11437
Anti-PSMA5 antibody used in Western Blot (WB). GTX11437

Cat. No. GTX11437

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Hamster
Package
50 μg ($399)
See all PSMA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 3 μg/ml
ICC/IF 2 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C A(120) L Q F G E E D A D P G A M(133) of human proteasome 20S subunit alpha 5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit alpha 5 , PSC5 , ZETA

Cellular Localization

Cytoplasm,Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-PSMA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11437

GTX11437 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX11437 PSMA5 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-PSMA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11437

GTX11437 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX11437 PSMA5 antibody.

Anti-PSMA5 antibody used in Western Blot (WB). GTX11437

GTX11437 WB Image

WB analysis of HeLa cell extract using GTX11437 PSMA5 antibody.
Lane 1 : Primary antibody
Lane 2 : Primary antibody pre-incubated with peptide

Anti-PSMA5 antibody used in Western Blot (WB). GTX11437

GTX11437 WB Image

WB analysis of whole cell extracts (30 μg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), Neuro-2a (Lane 3), Caki-1 (Lane 4), NTERA-2 (Lane 5), HCT 116 (Lane 6), Caco-2 (Lane 7), and tissue extract (30 μg lysate) of Mouse Liver (Lane 8) using GTX11437 PSMA5 antibody.
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399