*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , mouse brain
Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human PSMA6. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
proteasome subunit alpha 6 , IOTA , PROS27 , p27K
Cytoplasm , Nucleus
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]
GTX105061 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha 6(GTX105061) antibody at 1:200 dilution.
GTX105061 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105061 diluted at 1:1000
GTX105061 WB Image
Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105061 diluted at 1:500