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PSMA6 antibody

Anti-PSMA6 antibody used in IHC (Whole mount) (IHC-Wm). GTX105061
Anti-PSMA6 antibody used in IHC (Whole mount) (IHC-Wm). GTX105061
Anti-PSMA6 antibody used in IHC (Whole mount) (IHC-Wm). GTX105061
Anti-PSMA6 antibody used in Western Blot (WB). GTX105061
Anti-PSMA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105061
Anti-PSMA6 antibody used in Western Blot (WB). GTX105061

Cat. No. GTX105061

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PSMA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HepG2 , Mouse brain

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMA6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951509

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit alpha 6 , IOTA , PROS27 , p27K

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSMA6 antibody used in IHC (Whole mount) (IHC-Wm). GTX105061

GTX105061 IHC-Wm Image

PSMA6 antibody detects PSMA6 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
PSMA6 antibody (GTX105061) dilution: 1:100.
Anti-PSMA6 antibody used in IHC (Whole mount) (IHC-Wm). GTX105061

GTX105061 IHC-Wm Image

Immunofluorescent image of a section of the lens of a zebrafish embryo eye using psma6a antibody (GTX105061) at a 1:200 dilution.
Green: PSMA6
Red: Actin
(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-PSMA6 antibody used in IHC (Whole mount) (IHC-Wm). GTX105061

GTX105061 IHC-Wm Image

PSMA6 antibody detects PSMA6 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
PSMA6 antibody (GTX105061) dilution: 1:100.
Anti-PSMA6 antibody used in Western Blot (WB). GTX105061

GTX105061 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105061 diluted at 1:500

Anti-PSMA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105061

GTX105061 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha 6(GTX105061) antibody at 1:200 dilution.

Anti-PSMA6 antibody used in Western Blot (WB). GTX105061

GTX105061 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105061 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169