Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113531 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
12% SDS PAGE
GTX113531 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using proteasome alpha 7(GTX113531) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA7 antibody [N1C2] (GTX113531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.