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PSMA7 antibody [N1C3]

Anti-PSMA7 antibody [N1C3] used in Western Blot (WB). GTX101745
Anti-PSMA7 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101745
Anti-PSMA7 antibody [N1C3] used in Western Blot (WB). GTX101745
Anti-PSMA7 antibody [N1C3] used in Western Blot (WB). GTX101745

Cat No. GTX101745

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

proteasome subunit alpha 7 , C6 , HEL-S-276 , HSPC , RC6-1 , XAPC7

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PSMA7 antibody [N1C3] used in Western Blot (WB). GTX101745

GTX101745 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101745 diluted at 1:1000

Anti-PSMA7 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101745

GTX101745 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using proteasome alpha 7(GTX101745) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PSMA7 antibody [N1C3] used in Western Blot (WB). GTX101745

GTX101745 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
12% SDS PAGE
GTX101745 diluted at 1:1000

Anti-PSMA7 antibody [N1C3] used in Western Blot (WB). GTX101745

GTX101745 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA7 antibody [N1C3] (GTX101745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169