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PSMB10 antibody, Internal

Anti-PSMB10 antibody, Internal used in Western Blot (WB). GTX88408
Anti-PSMB10 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88408

Cat. No. GTX88408

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PSMB10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Tonsil shows efined staining in a subset of cells.
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88408-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PTEPVKRSGRYH, from the internal region of the protein sequence according to NP_002792.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit beta 10 , LMP10 , MECL1 , beta2i

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PSMB10 antibody, Internal used in Western Blot (WB). GTX88408

GTX88408 WB Image

WB analysis of human lung lysate using GTX88408 PSMB10 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PSMB10 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88408

GTX88408 IHC-P Image

IHC-P analysis of human tonsil using GTX88408 PSMB10 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399