GeneTex
United States (US)

PSMB10 antibody, Internal

GTX88408_976_IHC-P_18122102_463.jpg
GTX88408_2362_WB_18122102_220.jpg

Cat No. GTX88408

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 5-10μg/ml
IHC Assay dependent

Note :

Human Tonsil shows efined staining in a subset of cells.
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88408-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-PTEPVKRSGRYH, from the internal region of the protein sequence according to NP_002792.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BETA2I Antibody , LMP10 Antibody , MECL1 Antibody

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88408_976_IHC-P_18122102_463.jpg

GTX88408 IHC-P Image

IHC-P analysis of human tonsil using GTX88408 PSMB10 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml

GTX88408_2362_WB_18122102_220.jpg

GTX88408 WB Image

WB analysis of human lung lysate using GTX88408 PSMB10 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer