GeneTex
United States (US)

PSMB10 antibody

GTX32818_20180530_WB_18121410_524.jpg
GTX32818_20180530_IHC-P_1_18121410_747.jpg
GTX32818_20180530_ICCIF_18121410_982.jpg
GTX32818_20180530_IHC-P_18121410_240.jpg

Cat No. GTX32818

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

29 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proteasome Subunit Beta 10,Lmp10,Mecl1,Beta2I,Psmb10

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32818_20180530_WB_18121410_524.jpg

GTX32818 WB Image

WB analysis of THP-1 cell lysates using GTX32818 PSMB10 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32818_20180530_IHC-P_1_18121410_747.jpg

GTX32818 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32818 PSMB10 antibody.
Dilution : 1:200

GTX32818_20180530_ICCIF_18121410_982.jpg

GTX32818 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32818 PSMB10 antibody.
Blue : DAPI

GTX32818_20180530_IHC-P_18121410_240.jpg

GTX32818 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32818 PSMB10 antibody.
Dilution : 1:200

Package List Price ($)
$ 319