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PSMB6 antibody

Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331
Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331
Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331
Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331
Anti-PSMB6 antibody used in Western Blot (WB). GTX23331

Cat No. GTX23331

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Bovine
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/ml
ICC/IF 1:10-1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome (prosome, macropain) subunit, beta type 6 , Lmp19 , Mpnd

Cellular Localization

Cytoplasm,Nucleus

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman’s syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).

Database

Research Area

DATA IMAGES

Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331

GTX23331 ICC/IF Image

ICC/IF analysis of Hela cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331

GTX23331 ICC/IF Image

ICC/IF analysis of A431 cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331

GTX23331 ICC/IF Image

ICC/IF analysis of BAEC cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-PSMB6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23331

GTX23331 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 5 μg/ml for 1 hour at room temperature

Anti-PSMB6 antibody used in Western Blot (WB). GTX23331

GTX23331 WB Image

WB analysis of 25 ug of Hela (lane 1) and BAEC (lane 2) cell lysates using GTX23331 PSMB6 antibody.
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339