APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/ml |
1:10-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58)
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proteasome (prosome, macropain) subunit, beta type 6 , Lmp19 , Mpnd
Cellular Localization
Cytoplasm,Nucleus
Background
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman’s syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).
Database
Research Area
DATA IMAGES
|
GTX23331 ICC/IF Image
ICC/IF analysis of BAEC cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4ºC
|
|
GTX23331 ICC/IF Image
ICC/IF analysis of A431 cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4ºC
|
|
GTX23331 ICC/IF Image
ICC/IF analysis of Hela cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4ºC
|
|
GTX23331 WB Image
WB analysis of 25 ug of Hela (lane 1) and BAEC (lane 2) cell lysates using GTX23331 PSMB6 antibody. Dilution : 1:1000
|
|
GTX23331 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX23331 PSMB6 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 5 μg/ml for 1 hour at room temperature
|
REFERENCE
There are currently no references for PSMB6 antibody (GTX23331). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSMB6 antibody (GTX23331). Be the first to share your experience with this product.