APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human PSMB8 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proteasome subunit beta 8 , ALDD , D6S216 , D6S216E , JMP , LMP7 , NKJO , PRAAS1 , PSMB5i , RING10
Cellular Localization
Cytoplasm,Nucleus
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative t
Database
Research Area
DATA IMAGES
![Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486](/templates/default/js/lightbox/images/loading.gif "Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486")
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GTX60486 WB Image
WB analysis of HeLa (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate using GTX60486 PSMB8 antibody [1A5].
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GTX60486 IHC-P Image
IHC-P analysis of human colon tissue using GTX60486 PSMB8 antibody [1A5].
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GTX60486 IHC-P Image
IHC-P analysis of intima cancer tissue using GTX60486 PSMB8 antibody [1A5].
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GTX60486 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60486 PSMB8 antibody [1A5].
Green : PSMB8
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60486 WB Image
WB analysis of human PSMB8 (AA: 1-272) recombinant protein using GTX60486 PSMB8 antibody [1A5].
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GTX60486 ELISA Image
ELISA analysis of antigen using GTX60486 PSMB8 antibody [1A5].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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REFERENCE
There are currently no references for PSMB8 antibody [1A5] (GTX60486). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PSMB8 antibody [1A5] (GTX60486). Be the first to share your experience with this product.
![Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486](/upload/website/prouct_img/small/GTX60486/GTX60486_WB_w_23061123_265.webp "Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486")
![Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486](/upload/website/prouct_img/small/GTX60486/GTX60486_20170912_IHC-P_w_23061123_142.webp "Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486")
![Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486](/upload/website/prouct_img/small/GTX60486/GTX60486_20170912_IHC-P_1_w_23061123_523.webp "Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486")
![Anti-PSMB8 antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60486](/upload/website/prouct_img/small/GTX60486/GTX60486_20170912_ICCIF_w_23061123_947.webp "Anti-PSMB8 antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60486")
![Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486](/upload/website/prouct_img/small/GTX60486/GTX60486_20170912_WB_1_w_23061123_688.webp "Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486")
![Anti-PSMB8 antibody [1A5] used in ELISA (ELISA). GTX60486](/upload/website/prouct_img/small/GTX60486/GTX60486_20170912_ELISA_w_23061123_963.webp "Anti-PSMB8 antibody [1A5] used in ELISA (ELISA). GTX60486")
![Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486](/upload/website/prouct_img/normal/GTX60486/GTX60486_WB_w_23061123_265.webp "Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486")
![Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486](/upload/website/prouct_img/normal/GTX60486/GTX60486_20170912_IHC-P_w_23061123_142.webp "Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486")
![Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486](/upload/website/prouct_img/normal/GTX60486/GTX60486_20170912_IHC-P_1_w_23061123_523.webp "Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486")
![Anti-PSMB8 antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60486](/upload/website/prouct_img/normal/GTX60486/GTX60486_20170912_ICCIF_w_23061123_947.webp "Anti-PSMB8 antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60486")
![Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486](/upload/website/prouct_img/normal/GTX60486/GTX60486_20170912_WB_1_w_23061123_688.webp "Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486")
![Anti-PSMB8 antibody [1A5] used in ELISA (ELISA). GTX60486](/upload/website/prouct_img/normal/GTX60486/GTX60486_20170912_ELISA_w_23061123_963.webp "Anti-PSMB8 antibody [1A5] used in ELISA (ELISA). GTX60486")