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PSMB8 antibody [1A5]

Anti-PSMB8 antibody [1A5] used in ELISA (ELISA). GTX60486
Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486
Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486
Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486
Anti-PSMB8 antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60486
Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486

Cat No. GTX60486

Host

Mouse

Clonality

Monoclonal

Clone Name

1A5

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PSMB8 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome subunit beta 8 , ALDD , D6S216 , D6S216E , JMP , LMP7 , NKJO , PRAAS1 , PSMB5i , RING10

Cellular Localization

Cytoplasm,Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative t

Database

Research Area

DATA IMAGES

Anti-PSMB8 antibody [1A5] used in ELISA (ELISA). GTX60486

GTX60486 ELISA Image

ELISA analysis of antigen using GTX60486 PSMB8 antibody [1A5].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486

GTX60486 IHC-P Image

IHC-P analysis of human colon tissue using GTX60486 PSMB8 antibody [1A5].

Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486

GTX60486 WB Image

WB analysis of human PSMB8 (AA: 1-272) recombinant protein using GTX60486 PSMB8 antibody [1A5].

Anti-PSMB8 antibody [1A5] used in IHC (Paraffin sections) (IHC-P). GTX60486

GTX60486 IHC-P Image

IHC-P analysis of intima cancer tissue using GTX60486 PSMB8 antibody [1A5].

Anti-PSMB8 antibody [1A5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60486

GTX60486 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60486 PSMB8 antibody [1A5].
Green : PSMB8
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-PSMB8 antibody [1A5] used in Western Blot (WB). GTX60486

GTX60486 WB Image

WB analysis of HeLa (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate using GTX60486 PSMB8 antibody [1A5].

REFERENCE

There are currently no references for PSMB8 antibody [1A5] (GTX60486). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PSMB8 antibody [1A5] (GTX60486). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339