*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88410-PEP).
This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-DVSDLLHQYREANQ, from the C Terminus of the protein sequence according to NP_004150.1; NP_683720.2.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
proteasome subunit beta 8 , ALDD , D6S216 , D6S216E , JMP , LMP7 , NKJO , PRAAS1 , PSMB5i , RING10
Cytoplasm , Nucleus
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
GTX88410 IHC-P Image
IHC-P analysis of human spleen using GTX88410 PSMB8 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
GTX88410 WB Image
WB analysis of human liver lysate using GTX88410 PSMB8 antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer