GeneTex
United States (US)

PSMB8 antibody, C-term

GTX88410_975_IHC-P_18122102_958.jpg
GTX88410_2361_WB_18122102_413.jpg

Cat No. GTX88410

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88410-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DVSDLLHQYREANQ, from the C Terminus of the protein sequence according to NP_004150.1; NP_683720.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proteasome Subunit Beta 8,Aldd,D6S216,D6S216E,Jmp,Lmp7,Nkjo,Praas1,Psmb5I,Ring10,Psmb8

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative t

Database

Research Area

DATA IMAGES
GTX88410_975_IHC-P_18122102_958.jpg

GTX88410 IHC-P Image

IHC-P analysis of human spleen using GTX88410 PSMB8 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88410_2361_WB_18122102_413.jpg

GTX88410 WB Image

WB analysis of human liver lysate using GTX88410 PSMB8 antibody, C-term.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329