PSMB8 antibody [N2C3]

Name: PSMB8 antibody [N2C3]
Brand: GeneTex
SKU: GTX113533
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-PSMB8 antibody [N2C3] used in Western Blot (WB). GTX113533

Anti-PSMB8 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113533

Anti-PSMB8 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113533

Cat. No. GTX113533

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all PSMB8 products

View product citations for antibody GTX113533 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Sheep, Bovine, Dog, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMB8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037789

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit beta 8 , ALDD , D6S216 , D6S216E , JMP , LMP7 , NKJO , PRAAS1 , PSMB5i , RING10

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq]

Database

Gene ID: 5696 PSMB8
UniProt: P28062 PSMB8

Research Area

DATA IMAGES

GTX113533 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX113533 diluted at 1:1000

GTX113533 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PSMB8(GTX113533) antibody at 1:200 dilution.

GTX113533 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSMB8(GTX113533) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113533 IHC-P Image

PSMB8 antibody [N2C3] detects PSMB8 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PSMB8 antibody [N2C3] (GTX113533) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Gu Y et al. Blood Cancer J 2020; 10 (12) : 125 Downregulation of PA28α induces proteasome remodeling and results in resistance to proteasome inhibitors in multiple myeloma.

Application : WB

Reactivity : Human

Lee JA et al. Neurochem Int 2017; 112 : 96-107 Activation of the Nrf2 signaling pathway and neuroprotection of nigral dopaminergic neurons by a novel synthetic compound KMS99220.

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