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PSMB8 antibody [N2C3]

Anti-PSMB8 antibody [N2C3] used in Western Blot (WB). GTX113533
Anti-PSMB8 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113533
Anti-PSMB8 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113533
Anti-PSMB8 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113533

Cat No. GTX113533

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A431 , Molt-4 , Raji

Predict Reactivity

Sheep, Bovine, Dog, Pig, Japanese Medaka, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PSMB8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037789

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome subunit beta 8 , ALDD , D6S216 , D6S216E , JMP , LMP7 , NKJO , PRAAS1 , PSMB5i , RING10

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PSMB8 antibody [N2C3] used in Western Blot (WB). GTX113533

GTX113533 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX113533 diluted at 1:1000

Anti-PSMB8 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113533

GTX113533 IHC-P Image

PSMB8 antibody [N2C3] detects PSMB8 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PSMB8 antibody [N2C3] (GTX113533) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSMB8 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113533

GTX113533 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using PSMB8(GTX113533) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PSMB8 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113533

GTX113533 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PSMB8(GTX113533) antibody at 1:200 dilution.

REFERENCE

REVIEW

PSMB8 antibody [N2C3] Cat No. GTX113533

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 25-Aug-2016
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat brain tissue
Amount used : 25μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169